FIG. 1.

IKK target sequences, p105 point mutants, and synthetic phosphopeptides. (A) Schematic representation of mammalian NF-κB1 p105. The relative positions of the Rel homology domain (RHD), nuclear localizing signal (NLS), glycine-rich region (GRR), ankyrin repeats (vertical rectangles), death domain (DD), and PEST domain are shown. An alignment of the IKK target sequence in the p105 PEST region with those in IκBα, IκBβ, and IκBɛ is also shown (all human proteins). (B) Serine-to-alanine point mutants of HA-p105. WT, wild type. (C) Sequences of synthetic p105 and IκBα peptides. P, phosphorylated residue.