FIG. 10.

Effect of rosiglitazone on mitochondrial protein composition. On day 8 of differentiation, 3T3-L1 adipocytes were either left untreated (−) or were treated with 1 μM rosiglitazone (Rosi) for 24 h (+). Adipocytes were fractionated, and the mitochondria were isolated and solubilized in CHAPS. Equal amounts of mitochondrial protein were subjected to velocity centrifugation on 10 to 30% sucrose gradients, and gradients were fractionated into 10 fractions from the top. The gradient fractions were resolved side by side on a 7.5 to 15% polyacrylamide gel, which was stained with Sypro Ruby. Some of the more abundant bands exhibiting differences between untreated and treated states in two successive experiments (comparison not illustrated) were excised from the gel and subjected to peptide fingerprinting and database searching. The identities and gene identifier numbers from the selected bands are indicated.