FIG. 2.
PCR analysis of the Hnf-1α gene structure. (A) Structure of the Hnf-1α alleles after Cre-mediated DNA recombination and the PCR genotyping strategy. The PCR primer design for genotyping the targeted and recombined Hnf-1α alleles is shown. (B) Results of PCR analysis of the Hnf-1α alleles in tissues of 6-week-old Hnf-1αkin/kin mice. The PCR products were analyzed in ethidium bromide-stained 2% agarose gel. Symbols and abbreviations: +, wild-type allele; −, HNF-1α-null allele; kin, PGK.neo knockin HNF-1α allele.