FIG. 5.

Proteasome inhibitor MG115 does not influence early TRAIL-induced caspase 8, caspase 10, and cFLIP cleavage. Primary keratinocytes were incubated for 60 min with 10 μM MG115 or diluent alone and subsequently treated with 50 ng of TRAIL per ml for the indicated times. TRAIL treatment led to cleavage of caspase 8 (p43/p41/p18), caspase 10 (p47/43/25), and cFLIP (p43) under both conditions, as determined by Western blot analysis. Membranes were rehybridized with antitubulin monoclonal antibodies to confirm comparable loading of proteins.