FIG. 2.

Leukocyte recruitment and degranulation in grancalcin-deficient mice. (A and B) Neutrophil (A) and macrophage (B) recruitment in sterile peritonitis. PMN granulocytes and macrophages were obtained by peritoneal lavage at the time points indicated after injection of thioglycolate and identified by flow cytometry as Ly-6G- (Gr-1) or F4/80-positive cells, respectively (n = 3). (C) Degranulation of purified bone marrow neutrophils, as indicated by release of the secondary granule component lactoferrin upon a 30-min incubation at 37°C with or without additional stimulation. One hundred percent release corresponds to approximately 300 ng of lactoferrin/ml. PMA, phorbol-12-myristate-13-acetate.