TABLE 1.

Summary of FCS experiments

Cells (sample no.)	Site	Mean fluorescent-cell count/molecule ± SEM	Mean fluorescent-cell concn ± SEM (nM)	Mean diffusion constant ± SEM (cm2/s)		Mean % ± SEM
				Fast	Slow
GFP (n = 9)b	Nucleus	18 ± 0.7a	472 ± 82a	(2.1 ± 0.1) × 10−7		100
	Cytosol	21 ± 0.4	327 ± 37	(2.2 ± 0.1) × 10−7		100
GFP-CK2α (n = 40)c	Nucleus	14 ± 0.6a	252 ± 22a	(2.7 ± 0.2) × 10−7		47 ± 1a
					(1.5 ± 0.1) × 10−8a	53 ± 1a
	Cytosol	16 ± 0.7	111 ± 12	(2.4 ± 0.1) × 10−7		54 ± 2
					(1.8 ± 0.1) × 10−8	47 ± 2
GFP-CK2β (n = 40)c	Nucleus	15 ± 0.8a	333 ± 29a	(2.5 ± 0.2) × 10−7		42 ± 1
					(1.6 ± 0.1) × 10−8a	56 ± 1
	Cytosol	17 ± 0.9	185 ± 15	(2.7 ± 0.2) × 10−7		43 ± 2
					(2.2 ± 0.2) × 10−8	57 ± 2

^aNucleus-cytoplasm significant difference (α = 0.05).

^bOne-component fitting.

^cTwo-component fitting.