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The ade2-1 transcript is a substrate for NMD. Total RNA isolated from yeast strains with the indicated UPF/NMD genotypes was analyzed by Northern blotting with DNA probes that detected the ade2-1 or CYH2 transcripts. WT, wild type. The yeast strains used for this experiment were grown in YEPD at 30°C and included HFY1200 (WT), HFY870 (upf1Δ), HFY1300 (nmd2Δ), HFY861 (upf3Δ), and HFY1067 (dcp1Δ).
