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Galactose-inducible expression of UPF1, NMD2, and UPF3. Yeast strains harboring upf1Δ (HFY870), nmd2Δ (HFY1300), or upf3Δ (HFY861) mutations and the respective, complementary galactose-inducible NMD gene construct were grown in SC raffinose liquid medium without uracil to mid-log phase (OD600 = 0.5). Galactose was then added to a final concentration of 2%, culture aliquots were taken at 10-min intervals, and all samples were subsequently analyzed by Western blotting. Gal., galactose.
