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t1/2 of the ade2-1 mRNA in SC-galactose medium. Yeast cells (HFY456) harboring nmd2Δ and rpb1-1 mutations were grown in SC-galactose medium at 24°C and then shifted to 37°C to inhibit further transcription. RNA was isolated at different times after the temperature shift and subjected to Northern blotting as in Fig. 1. Hybridization to the SCR1 probe served as a loading control (52).
