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. 2003 Feb;23(3):842–851. doi: 10.1128/MCB.23.3.842-851.2003

FIG. 4.

FIG. 4.

The ade2-1 transcript is rapidly degraded upon activation of NMD. (A) Activation of NMD causes rapid degradation of ade2-1 mRNA. Total RNA was isolated from yeast strains (HFY870, HFY1300, and HFY861) harboring upf1Δ, nmd2Δ, or upf3Δ mutations and the respective, complementary galactose-inducible NMD gene constructs (GAL-UPF1, GAL-NMD2, or GAL-UPF3). Galactose induction was performed as described in Fig. 2, and RNA was analyzed by Northern blotting with a DNA probe that detected the ade2-1 transcript. (B) The addition of galactose does not destabilize ade2-1 mRNA. Total RNA isolated from yeast strains with the indicated UPF/NMD genotypes, and harboring only the vector plasmid (GAL-vector), was analyzed by Northern blotting as in Fig. 4A. Gal., galactose.