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. 2003 Feb;23(3):842–851. doi: 10.1128/MCB.23.3.842-851.2003

FIG. 5.

FIG. 5.

FIG. 5.

Degradation of the ade2-1 mRNA occurs on polysomes. (A) The ade2-1 mRNA is detected in the polysome fractions before galactose induction. Total RNA isolated from polysome fractions collected before the addition of galactose was analyzed by Northern blotting with DNA probes that detected the ade2-1 mRNA and the SCR1 RNA (the latter to serve as a control to ensure that RNA was isolated from the polysome fractions). (B) The ade2-1 mRNA is no longer detected in the polysome fractions upon activation of NMD. Total RNA isolated from polysome fractions collected 30 min after the addition of galactose was analyzed by Northern blotting as described for panel A. The results depicted in this figure were obtained from the upf1Δ yeast strain (HFY870) harboring the galactose-inducible UPF1 construct.