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. 2003 Feb;23(3):842–851. doi: 10.1128/MCB.23.3.842-851.2003

FIG. 6.

FIG. 6.

pgk1 mRNA harboring an early nonsense codon degrades rapidly upon activation of NMD. (A) Activation of NMD results in degradation of a pgk1 transcript containing an early nonsense codon but does not destabilize a pgk1 transcript with a late nonsense codon. Total RNA isolated from yeast strains (HFY870, HFY1300, and HFY861) with the indicated UPF/NMD genotypes harboring complementing galactose-inducible NMD gene constructs (GAL-UPF1, GAL-NMD2, or GAL-UPF3) was analyzed by Northern blotting with a DNA probe that detected nonsense-containing pgk1 transcripts. (B) The addition of galactose does not destabilize pgk1 mRNA harboring an early nonsense codon. Total RNA isolated from yeast strains with the indicated UPF/NMD genotypes harboring only the vector plasmid (GAL-vector) was analyzed by Northern blotting as described for panel A. Gal., galactose.