FIG. 1.

Schematic and sequence of the MMTV Wt and mutant promoter constructs. (A) Schematic of Wt and LS mutant constructs. In the wild-type pMMTV-CAT construct the MMTV promoter is fused to the coding region of a CAT gene, and the wild-type construct pLS-Wt and the LS mutant promoters are fused to the coding region of the herpesvirus TK gene. (B) Sequence of Wt and the LS mutant constructs. The LS mutant constructs used in this work include promoter constructs with mutations in the NF1 binding site (half site; mNF1), octamer binding site (mOTF), and proximal (mPhre) and distal (mDhre) HREs. The top line of the sequence represents wild-type sequences and the bottom line shows mutant sequences. Gaps in the mutant sequences denote deletions, and bold characters denote mismatches.