FIG. 4.

Expression from a transient MMTV template in the absence of NF1 binding is reduced. MMTV constructs (pLS-Wt and LS mutant constructs) along with a pGL3-control plasmid were transfected into C127 cells for 24 h. The cells were treated with DEX for 4 h and RNA was isolated. (A) Primer extension was performed with 100 μg of total RNA with oligo-22 and with 2 μg of RNA with an 18S-specific primer. For transfection efficiency, message from pGL3-control (luciferase) was analyzed by PCR with primer sets (GLR and GL2) using first-strand cDNA. The products were run on 8% denaturing polyacrylamide gels.