FIG. 8.

BRG1 and GR do not associate with the promoter in the absence of NF1 binding. Cells from stable cell lines containing Wt (pLS-Wt) or mNF1 constructs were treated without or with DEX for 1 h and analyzed by ChIP assay with BRG1 and GR antibodies. The cells were sonicated and immunoprecipitated with nonspecific (rabbit IgG) or BRG1 antibodies (A) or nonspecific (mouse IgG) or GR antibodies (C). The MMTV promoter sequence was detected by PCR using oligo-38 and ³²P-end-labeled oligo-22. The PCR products were run on nondenaturing polyacrylamide gels and exposed to PhosphorImager screens for further analysis. The association of BRG1 (B) or GR (D) with Wt and NF1 templates was quantified from two independent experiments and by setting the association with the wild-type promoter in the presence of DEX to 100.