FIG. 6.

In vivo association of αCP with polysome-bound hα-globin mRNA. Equal amounts of the prepolysomal (S130) and polysomal (P) fractions of K562 cytoplasmic extracts were individually immunoprecipitated with an antibody specific to the αCP2 and αCP2-KL isoforms. RNA extracted from the immunoprecipitates was assessed for specific mRNA content by RPA. This study was carried out independently four times with consistent results. (A) RPA using probes to hα-globin and GAPDH mRNAs. (B) RPA using probes to hα-globin and γ-globin mRNAs.