Fig. 7. Heteromeric interaction between Eag1 and Erg1:Eag1TCC. Currents were obtained in oocytes expressing wild-type Erg1, wild-type Eag1 or the chimera Erg1:Eag1TCC alone (upper traces), or co-expressing Eag1 + Erg1 or Eag1 + Erg1:Eag1TCC (lower traces). Current traces were obtained using a protocol designed to highlight both Eag1 and HERG properties which consisted of a long (5 s) preconditioning pulse to –140  mV (black traces) or –60 mV (red traces) followed by a depolarizing pulse to +60 mV for 1 s and a depolarization to –120 mV lasting for 1.5 s, and are shown after normalization to the maximal current value. Note the strong voltage dependence of the activation in Eag1 which is abolished in oocytes injected with Eag1+Erg1:Eag1TCC.