Fig. 6. Constitutively active rhodopsin in the ninaE^pp100 mutant is associated with localization of DGqα to the cytosol. In the ninaE^pp100 mutant, rhodopsin constitutively activates DGqα. The percentage of DGqα in the cytosol of dark-adapted ninaE^pp100 flies (circles) is similar to that obtained after prolonged activation of the wild-type flies (squares). Illumination of the mutant flies does not promote further translocation of DGqα to the cytosol. The graph shows the mean amount of DGqα in the supernatant (as a percentage of total) ± SE from three independent experiments. The graph of wild-type flies is identical to Figure 1A and is shown for comparison.