Fig. 7. Severe reduction of Gβ inhibits the translocation of DGqα and abolishes its recovery. (A) The G_βe¹ mutant contains low levels of DGqβ. Only 50% of DGqα is localized to the membranes of G_βe¹ dark-adapted flies as compared with 80% in wild-type flies (Figure 1). In contrast to the massive initial translocation observed in wild-type flies, in G_βe¹ flies there is an obvious lag in the translocation kinetics of DGqα The graph shows the mean amount of DGqα in the supernatant (as a percentage of total) ± SE from four independent experiments. (B) G_βe¹ flies were illuminated for 60 min and then incubated in the dark for various times. Even prolonged incubation of G_βe¹ flies in the dark (2 h) produced no discernible recovery. The graph shows the mean amount of DGqα in the supernatant (as a percentage of total) ± SE from four independent experiments.