Fig. 5. Interactions between nexins. Yeast strains deleted for the appropriate gene or pair of genes were transformed with plasmids expressing the corresponding nexins, one protein A tagged and one HA tagged, as indicated. The protein A-tagged form was isolated, and co-purifying HA-tagged protein detected by immunoblotting (arrowheads). The immunoprecipitates (IP) contain more cell equivalents than the extracts (Ex), but the ratio is the same in each case. The band indicated with an asterisk represents binding of the anti-HA antibody to Snx42p– protein A.