Fig. 7. The Snx4 family of nexins have functions distinct from that of retromer. (A) Detection of carboxypeptidase Y secreted by each sorting nexin mutant. Note that only vps5, vps17 (both encoding retromer subunits) and mvp1 show a detectable phenotype. (B) Synthetic growth defect. The indicated mutants, in two strain backgrounds, were grown to saturation in liquid culture and then spotted at high and low concentrations on plates.