Fig. 5. Communication between ATPase and DNA-binding domains. (A) ADP binding in the absence or presence of homoduplex (GC) or heteroduplex (GT) DNA for wild-type MutS. (B) ADP binding for R697A MutS in the presence of DNA. (C) Relative amounts of DNA bound to MutS in the absence or presence of ADP or ATPγS. DNA bound by wild-type MutS in the absence of nucleotides was set to 100%.