Table I. ADP binding and ATP hydrolysis.
Kd1 (µM) | Kd2 (µM) | Km (µM) | kcat (/min) | Hill coefficient | Inhibitiona | |
---|---|---|---|---|---|---|
Wild type | <0.1 | 10.3 ± 1.8 | 8.7 ± 1.0 | 13.2 ± 0.8 | 1.08 | 90% |
R697A | 9.6 ± 2.0 | 9.6 ± 2.0 | 5.1 ± 0.7 | 1.1 ± 0.1 | 1.04 | 50% |
aInhibition of ATP hydrolysis at an equal molar ratio of ATP and AMPPNP.