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| Kd-GT (µM) | Kd-GC (µM) | Kd-GC/Kd-GT | ATP releasea (%) | Rifampicin resistanceb | |
|---|---|---|---|---|---|
| Wild type | 0.44 ± 0.05 | 9.5 ± 0.9 | 21.6 | 4 | 0.2 ± 3.8 |
| R697A | 0.92 ± 0.10 | 17.8 ± 1.9 | 19.3 | 25 | 45 ± 12 |
| E694N | – | – | – | – | 45 ± 15 |
| mutS– | – | – | – | – | 29 ± 4 |
aRelative amount of DNA bound in the presence of ATPγS when compared with the amount bound in the absence of nucleotides (Figure 5C).
bMedian number of rifampicin-resistant colonies per 1 × 108 cells plated out.
