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. 2003 Feb 3;22(3):657–667. doi: 10.1093/emboj/cdg066

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graphic file with name cdg066f2.jpg — Fig. 2. LC/MS nucleoside analysis of tRNA^Glu. (A and B) Chromatograms for cy tRNA^Glu and mt tRNA^Glu, respectively. Top: UV chromatograms for nucleosides. Bottom: mass chromatograms for modified uridines with mass filters at m/z 332.9 and 636.9 to detect, respectively, mcm⁵s²U in cy tRNA^Glu and a dimer form of mcm⁵Um with the adjacent uridine (mcm⁵UmpU) in mt tRNA^Glu. (C and D) Mass spectra for (C) mcm⁵s²U in cy tRNA^Glu and for (D) mcm⁵UmpU in mt tRNA^Glu. The chemical structure of each nucleoside is shown within the spectrum.