Table I. Effects of GSK3β mutations on immunoprecipitation of axin and FRAT.

Residue	Mutation	Axin binding (%)	Axin contacts	FRAT binding (%)	FRAT contacts
Y216	F	40	None	40	H200
Y216	E	100	None	100	H200
I228	K	5	F388	5	L203, L212
F229	Q	5	F388, L392	5	L212, I213
F229	A	10	F388, l392	5	L212, I213
V263	G	30	F388, E391, L392, R395	10	L203, L206, R219
L266	G	10	F388, L392	15	L212, A216
V267	G	0	L392, R395, L396, V399	100	A216, L220
I270	K	10	L392, L396	20	A216, L220
I270	G	0	None	0	A216, L220
I281	G	5	None	10	I213
N285	E	20	None	30	None
N285	D	25	None	50	None
Y288	R	5	P385, F388	10	V207, G210, N211, L212
Y288	F	100	P385, F388	20	V207, G210, N211, L212
Y288	A	70	P385, F388	5	V207, G210, N211, L212
E290	M	40	None	30	G210, N211, L212, I213, K214
E290	Q	100	None	10	G210, N211, L212, I213, K214
E290	D	80	None	10	G210, N211, L212, I213, K214
E290	K	80	None	40	G210, N211, L212, I213, K214
F291	L	10	Q386, A389	10	None
F293	A	5	A389, L392, I393, L396	20	I213
F293	Q	5	A389, L392, I393, L396	20	I213
I296	G	0	L396, Q400	20	V217, L220, H221
I296	T	0	L396, Q400	15	V217, L220, H221