Fig. 9. Other homeodomains utilize conserved eIF4E-binding sites to bind eIF4E. (A) The eIF4E-binding site in Hox11 is conserved from chicken to human. Conserved residues are highlighted in yellow. Accession numbers are: XP-046733 (human), P43345 (mouse), AA14453 (Xenopus) and 093366 (chicken). (B) Purified Hox11 binds to purified wild-type eIF4E with the same affinity as PML and PRH (Figure 2), binding ∼10% of eIF4E input. Hox11 and PML RING are immobilized GST fusion proteins, and were incubated with purified wild-type or mutant eIF4E, as indicated. Hox11Y45A mutation does not interact with eIF4E. Loading for Hox11 wild-type and mutants is shown by the Coomassie Blue gel (C.B. Hox11). W.B. indicates western blot.