Fig. 7. Reduced embryonic survival in hypoxia after NO scavenging. Eight- to 10-h wild-type embryos were made hypoxic and assayed for survival (black bars). In parallel, embryos were pre-incubated with the NO scavenger PTIO, made hypoxic, and assayed for survival (gray bars). Samples were incubated in normoxic or hypoxic conditions for 0, 17.5, 24 and 46 h, and then placed on an agar plate in normoxia, and scored for the number of embryos that hatched into larvae. The percentage of embryos that survived was 88.7 ± 1, 92.0 ± 4, 90.0 ± 2 and 55.3 ± 10% after 0 (normoxia), 17.5, 24 and 46 h of hypoxia, respectively. In contrast, 88.0 ± 3, 52.7 ± 4, 41.3 ± 2 and 1.3 ± 1% of PTIO-incubated embryos survived after equivalent periods of hypoxia.