FIG. 1.

Poliovirus RNAs utilized in this study. (A) Diagram of poliovirus PV1(A)₈₀ transcript RNA. Proteins encoded in the P1, P2, and P3 regions of the RNA are indicated. The locations of mutations used in this study are also shown. (B and C) Diagrams of the P23 and P3 expression RNAs. These RNAs contained the 5′ NTR, including the 5′ cloverleaf and IRES, an AUG initiation codon including Kozak's consensus sequence, the P23 (or P3) coding sequence, the 3′ NTR, and poly(A) tail. A 2BCP3 expression RNA was constructed using a similar strategy. (D) Diagram of a generic expression RNA containing the coding sequence for viral proteins from the P2 region of the viral genome. This RNA contains the 5′ cloverleaf and IRES, an AUG initiation codon, the coding sequence of one of the proteins listed in Table 1, two stop codons, the C-terminal end of the 3D coding sequence, the 3′ NTR, and poly(A) tail. The two stop codons were engineered at the end of the protein-coding sequence to terminate translation and block the translation of the C-terminal end of the 3D coding sequence.