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. 2003 Jan;77(2):1427–1440. doi: 10.1128/JVI.77.2.1427-1440.2003

FIG. 10.

FIG. 10.

Overexpression of Hrs (Vps27) restores CD4 surface expression in VV-M153R-transfected cells. HeLa cells cotransfected with CD4 (top left panel), CD4, Hrs, and GFP (top right panel), CD4, M153R, and GFP (bottom left panel), and CD4, Hrs, M153R, and GFP (bottom right panel) were analyzed by flow cytometry. Surface expression of CD4 was detected with anti-CD4 monoclonal antibody. CD4 staining is plotted against GFP fluorescence. Note that the CD4 control did not contain GFP. While CD4 is downregulated in VV-M153R-transfected cells, Hrs appears to reverse the effect of M153R in cells cotransfected with Hrs and M153R.