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. 2003 Jan;77(2):1427–1440. doi: 10.1128/JVI.77.2.1427-1440.2003

FIG. 2.

FIG. 2.

M153R downregulates MHC-I and CD4. HeLa or HeLa-CD4 cells infected with wild-type VV (light line), VV-K5 (left panel, dark line), or VV-M153R (right panel, dark line) were analyzed by flow cytometry. Surface expression of endogenous MHC-I was visualized with Cy-chrome-labeled monoclonal antibody W6/32. CD4 expression was achieved by coinfection with VV-CD4 or constitutive expression in HeLa-CD4 cells. Surface expression of CD4 was detected with anti-CD4 monoclonal antibody. TfR expression was detected with anti-TfR monoclonal antibody, and nontransfected cells served as a control (light line) in this experiment. Note that both CD4 and MHC-I are downregulated by M153R, whereas K5 downregulates MHC-I only. TfR is downregulated by neither M153R nor K5.