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. 2003 Jan;77(2):1427–1440. doi: 10.1128/JVI.77.2.1427-1440.2003

FIG. 7.

FIG. 7.

M153R requires lysine residues to downregulate CD4 and HLA-A2.1. (A) HeLa cells were infected with VV-CD4Δcyto in the presence (dark line) or absence (light line) of M153R. (B) (Top) HeLa cells were transfected with GFP together with CD4 only (left) or with CD4 and M153R (right). (Bottom) HeLa cells were transfected with GFP together with CD4KRcyto only (left) or with CD4KRcyto and M153R (right). Surface expression of CD4 was visualized by using anti-CD4 antibody together with PE-conjugated anti-mouse antibody. CD4 staining is plotted against GFP fluorescence. (C) The indicated HLA-A2.1 constructs were cotransfected into HeLa cells with GFP and either a vector control; K5PHD/LAP, a nonfunctional mutant of K5 (38); M153R-Flag/pUHD10.1; or K5/pUHD10.1. HLA-A2.1 surface expression was monitored by using HLA-A2.1-specific antibody BB7.2. HLA-A2.1 staining is plotted against GFP fluorescence. wt, wild type.