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Characterization of MPs synthesized by 72Irev and 126Srev. (Upper panel) Autoradiograph showing the in vivo phosphorylation of each virus. After metabolic labeling with 32P, MPs were immunoprecipitated from each lysate of infected protoplasts. Wild-type (Wt) ToMV (W3) and 37A238A were used as positive and negative controls to show phosphorylated MP, as analyzed previously (13). (Lower panel) Immunodetection of MPs by anti-ToMV MP serum (20).
