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. 2003 Jan;77(2):1382–1391. doi: 10.1128/JVI.77.2.1382-1391.2003

FIG. 6.

FIG. 6.

(A) Schematic diagram of the sequence of HSV-1(F) and of the recombinant virus YK304 and the BAC-excised recombinant virus derived from it. Line 1, sequence arrangement of the HSV-1(F) DNA. Line 2, location of the EcoRV-HindIII fragment used as a radiolabeled probe in panel B. Line 3, an enlarged portion of the domain of HSV-1(F) encoded by EcoRI J and D fragments. Line 5, sequence of the recombinant virus YK304. Line 7, sequence of the BAC-excised recombinant virus, which is generated by coinfection of Vero cells with YK304 and AxCANCre. Lines 4, 6, and 8, expected sizes of DNA fragments generated by cleavage of the DNA. The fragment designations shown here are identical to those used in the text and panel B. (B) Autoradiographic image of electrophoretically separated EcoRI digests of DNAs of YK304 (lane 1) or the BAC-excised recombinant virus (lane 2) hybridized to the radiolabeled EcoRV-HindIII fragment of pRB442. The letters on the right refer to the designations of the DNA fragments generated by restriction endonuclease cleavage. Molecular sizes are shown on the left.