FIG. 4.

Identification of GP regions containing B-cell epitopes by use of VSV pseudotyped with chimeric GPs. The numbers inside the bars are the amino acid positions of GP of Ebola virus species Zaire and Reston. All of the chimeric GPs were functional and complemented infectivity of VSVΔG*. For neutralization tests, viruses were incubated with appropriately diluted mouse ascites (50 μg of specific antibodies/ml) and inoculated onto 293 cells. The relative percentage of infected cells was determined by setting the number of infected cells in the presence of normal mouse ascites (approximately 50 to 100 GFP-positive cells per microscopic field) at 100. Experiments were done three times, and representative data are shown.