TABLE 1.
Primers used for PCR mutagenesis and genome sequencing
| Primer | Sequence (5′ to 3′)a | Sense | MHV-A59 spike gene locationb |
|---|---|---|---|
| JJ2 | cgcgaagcttgaatccTAGGGTATATTGGTGATTTTAGATGTATCC | + | 36-73 |
| RS366 | GCGGAAGCatcgatATTATTACAAAACAAAGACTCAGCCTG | − | 1096-1134 |
| JFS1 | ATGCTGTTCGTGTTTATTCTA | + | 1-21 |
| JRS414 | GCGCAGTCTGCAGAAAtccggaGTTACCAATCTGTAAATC | − | 1204-1241 |
| SS2 | GTATCAATCTTATAATTAGC | − | 1240-1259 |
| A59-5 | AAGTGTATGGCAGGTGCTTTGG | + | 1136-1157 |
| WZL-60B | GAACATAGCTGCCGCAGTAGC | − | 2791-2811 |
| A59-9 | TCTGATGTTGGCTTTGTCGAGG | + | 2647-2668 |
| WZL-01 | GGGGGATCCAGGTAGC | − | Intergenic region between S and 4a open reading frame |
a
Restriction sites introduced are denoted by lowercase letters.
b
The locations are designated by nucleotide positions in the spike (S) open reading frame.