FIG. 4.

Frequency distribution of the sizes of cloned 2-LTR circular vDNA PCR products from cells incubated with mutant and wild-type viruses. Inserts of the PCR product clones were analyzed for their sizes by either agarose gel electrophoresis or sequence analysis. Results are reported as the deviation from the expected size of 346 bp (Fig. 2). The frequencies were compiled in 20-nt windows from the expected size. Frequencies of the variance of insert sizes are reported as a percentage of the total number of samples examined. Distributions for wild-type (⧫) and IN_D116N (▪) (A), NC_H23C (⧫) and NC_H23C/IN_D116N (▪) (B), and NC_H44C (⧫) (C) virus infections are shown.