TABLE 4.
Quantitation of 2-LTR circular vDNA
| Virus | % of full junction with respect to total no. by:
|
|
|---|---|---|
| Quantitative PCRa | Cloning and sequencingb | |
| Wild type | 37 ± 5 | 43 |
| IND116N | 87 ± 9 | 91 |
| NCH23C | 54 ± 7 | 55 |
| NCH23C/IND116N | 58 ± 6 | 63 |
| NCH44C | 58 ± 3 | 61 |
a
Values expressed as percent ratio (mean ± standard deviation) of full junction/total 2-LTR circles from at least three individual transfection-infection experiments.
b
Percentage of full junctions/total 2-LTR junctions obtained on analysis of sequence data (Fig. 5), with correction for differential detection resulting from the use of primers that target slightly different regions of the expected circle junction sequence.