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. 2003 Jan;77(2):963–969. doi: 10.1128/JVI.77.2.963-969.2003

FIG. 2.

FIG. 2.

Truncation of HTLV-1 Env TM induces syncytium formation in NIH 3T3(TK−) cells. (A) Schematic representation of HTLV Env. The SU and TM subunits are indicated. The HTLV-1 Env signal peptide, fusion peptide, and TM membrane anchor are shown as dotted boxes, from the amino terminus to the carboxy terminus, respectively. Amino acid residues of the membrane anchor (anchor) and cytoplasmic domain (CD) are shown, and the position of the last carboxy-terminal residue of each mutant is indicated with an arrowhead. Amino acid residue numbering starts from the first signal peptide methionine of the HTLV-1 Env precursor. (B) Parental HTLV-1 Env (H) and truncation mutants lacking the 8 (HdC8) or 16 (HdC16) carboxy-terminal amino acids of the TM were tested for their ability to form syncytia after transfection in NIH 3T3 or NIH 3T3(TK−) cells. Open arrows in the panels on the right point to syncytia.