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. 2003 Jan;77(2):1337–1346. doi: 10.1128/JVI.77.2.1337-1346.2003

FIG. 1.

FIG. 1.

HIV-1, HIV-2, and SIV gp120 binding to CD4, chemokine receptors, or DC-SIGN(R). gp120 produced in 293T cells was bound to receptor expressing cells with or without sCD4 as indicated, and the bound protein was analyzed by SDS-PAGE and Western blot. Unbound gp120 is also shown in panels A and B (lanes labeled gp120). (A) HIV-1 gp120 proteins JR-FL and HXBc2 were bound to CD4, DC-SIGN, DC-SIGNR, or no receptor (pcDNA3). (B) HIV-2 gp120 proteins SBL/ISY and VCP were additionally evaluated on CXCR4. VCP, a CD4 independent Env, can bind directly to CXCR4, whereas SBL/ISY gp120 cannot. (C) SIVmac316 gp120 was evaluated on CCR5 or CCR5 with an aspartic acid at position 13 (CCR5/N13D), which confers more efficient binding of SIV gp120 (36).