FIG. 4.

Receptor binding and glycosylation characteristics of Env from viruses grown in T-cell lines, PBMCs, pr MDMs or in the presence of DMJ. Supernatant from HIV- or SIV-infected cells was used in receptor binding assays. Bound gp120 was analyzed by SDS-PAGE and Western blot. HIV-1, HIV-2, and SIV grown in T-cell lines (A), PBMCs (B), or MDMs (C) were bound to cells expressing CD4, DC-SIGN, DC-SIGNR, or no receptor. (D and E) HIV-1 Ba-L virus from PBMCs or MDMs (D) or SupT1/CCR5 cells (E) grown in the presence or absence of 2.5 mM DMJ (absence labeled as UnTx and presence labeled as DMJ) was digested with Endo H (H) or PNGase F (F) or left untreated (UnTx) and then analyzed by SDS-PAGE and Western blot for gp120.