FIG. 6.

Infection by Ebola virus GP pseudovirions and analysis of GP glycosylation. (A) 293T cells mock transfected or transfected with DC-SIGN, DC-SIGNR, or ASGP-R were infected with 500 TCID₅₀ of HIV-luciferase reporter viruses pseudotyped with VSV-G, EboZ-GP, EboS-GP, or EboZ-GP generated in the presence of 2.5 mM DMJ or MDM-derived EboZ-GP. Values are represented as the percent infection, calculated by using luciferase activity normalized to mock-transfected cells. Mean values plus the standard error of the mean are represented. (B) EboZ-GP and EboS-GP obtained from pseudovirions were incubated with the indicated lectin-biotin conjugates and then precipitated with streptavidin-agarose and analyzed by SDS-PAGE and Western blot for GP. Lectins are identified as follows: Vivia villosa lectin (VVL), Ricinus communiz agglutinin (RCA120), concanavalin A (ConA), Datura stramonium lectin (DSL), Erythrina cristagalli lectin (ECL), wheat germ agglutinin (WGA), Galanthus nivalis lectin (GNL), peanut agglutinin (PNA), Jacalin, and Ulex europaeus agglutinin (UEA). Unbound cell lysate is also shown.