FIG. 8.

Location of high-mannose or complex N-glycans on the HIV Env trimer. A model of the HIV Env trimer based on optimization of quantifiable surface parameters (30) is shown in three different views, each rotated 90° about a horizontal axis. The top panel is a view from the virus. The middle panel is a side view with the viral membrane above and the target cell below. The bottom panel is a view from the target cell. The left column is an α-carbon worm trace with gp120 in brown and CD4 in yellow. The protein proximal Man₃GlcNAc₂ pentasaccharide core conserved between high-mannose and complex N-glycans is shown in cyan and modeled as described previously (58). The right column depicts the solvent-accessible surface of gp120 with high-mannose N-glycans in blue, complex N-glycans in black (32), and the rest of the surface in white. (This figure was kindly provided by Peter D. Kwong.)