TABLE 1.

Primers used for PCR amplification

Primer	Sequence (5′→3′)a
K-bZIP NF	TAcggtccgaccATGCCCAGAATGAAGGA CATACC
K-bZIP 120F	CGggatccCTTCCAACTCGCAGATCCAA GAGGCGA
K-bZIP 122F	AAcggtccgACTCGCAGATCCAAGAGG CGA
K-bZIP 190F	AAcggtccgGCATTAGAAGAAAAGGATG CACAA
K-bZIP 122R	ATcggaccgAAGCTGTTGCGAAATGTGT GGTCC
K-bZIP 189R	AAcggaccgCTGCTGCAGCTGTCTTGTGTA
K-bZIP CR	ATcggaccgCAATAAACCCACAGCCCAT AG
K-bZIP TA-R	ctaatgcAAGCTGTTGCGAAATGTGTGG
K-bZIP LZ-F	acagcttGCATTAGAAGAAAAGGATGC ACAAC
K-Rta N-Cpo	TAcggtccgACCATGGCGCAAGATGACAA
K-Rta 110F	TAcggtccgATTATTCGGATCCTCACGG AG
K-Rta 239F	TAcggtccgATTACCACCGGCAAGGTCAC
K-Rta 299F	TAcggtccgAAACCCCATCCCAACATG
K-Rta 499F	TAcggtccgTGTAGAGATTCAACGGC
K-Rta 550F	TAcggtccgTTGGGATCAATTACCACCC
K-Rta 111R	TAcggaccgAATAATGCCTTGGGATGCCTC
K-Rta 240R	TAcggaccgGTAATTGGCCGGCGTTTCT
K-Rta 297R	TAcggaccgCTAATGACAAACTGGCTCA GG
K-Rta 503R	TAcggaccgTTGAATCTCTACACGGCAC AC
K-Rta 550R	TAcggaccgCAAAGAGGTACCAGGTGTC GT
K-Rta CR	TAcggaccgTCAGTCTCGGAAGTAATTAC GC
ORF57 promoter F	GAAgagctcCAAGACCATTAGCTATCTG CC
ORF57 promoter R	GAtagctaGCCTATTTTGGGAACCTGGC AG
PAN RNA promoter F	GAAgagctcGGACCGTGGGCGAGCCGA AATA
PAN RNA promoter R	GAtagctaGCTGGGCAGTCCCAGTGCTA AAC
K-Rta probe F	GTGCACGCCACGGATGTCGCCACG
K-Rta probe R	TCAGTCTCGGAAGTAATTACGCC
ORF57 probe F	GTTACCAGATTTAGATTACTTCATC
ORF57 probe R	CTTAAGAAAGTGGATAAAAGAATAA ACCC
PAN RNA probe F	GACTAAAGTGGTGTGCGGCAG
PAN RNA probe R	CAATCGACGCAAGTCAAGACAC
Probe S.B(unique).-F	CAAGAGTGCCATGTTTATATCTGC
Probe S.B(unique).-R	CTAATAAAGGATGGAAAACAGTCTG
Probe S.B(TR).-F	CCTGGTAGCCAGTACTTACCAATAAT TCC
Probe S.B(TR).-R	GGTGTTCACGTAGTGTCCAGGGCTC CAC

^aIn all sequences, the italic lowercase nucleotides represent restriction enzyme sites used for cloning the PCR products. The lower case nucleotides of primers K-bZIP TA-R and K-bZIP LZ-F show the overlapping region allowing the PCR products to anneal to each other for the K-bZIPΔBR basic region deletion mutant.