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. 2003 Jan;77(2):931–942. doi: 10.1128/JVI.77.2.931-942.2003

FIG. 7.

FIG. 7.

HIV-2 gp120 binding to CXCR4 with alanine substitutions in the N terminus, ECL1, ECL2, or ECL3. HIV-2 VCP or ROD/B gp120 produced in 293T cells was bound to QT6 cells expressing CXCR4, CXCR4 with alanine substitutions, or no receptor (pcDNA3). Cells were washed and lysed, and lysates were analyzed for bound gp120 by SDS-PAGE and Western blotting. VCP gp120 was evaluated without sCD4, while ROD/B gp120 was evaluated in the presence of 100 nM sCD4. The original amino acids replaced by alanine and their numerical positions are shown for the N terminus (A), ECL1 (B), ECL2 (C), and ECL3 (D).