FIG. 4.

F7F41S and F7F5 tandem fiber chimeras. (A) Diagram of chimeric viruses constructed using F7 inserted 5′ to either F41S or F5 terminal exons into the 3′ end of the MLTU. (B) Coomassie blue staining of SDS-polyacrylamide gel loaded with 5 × 10¹¹ virus particles of dlAd5NCAT (lane 2), F7F5 (lane 3), F7 (lane 4), (F7F41S) lane 5, or F4T (lane 6). Arrows indicate designated fiber polypeptides. (C) Northern blot analysis of 293 cell RNA isolated 24 h postinfection with 10³ particles/cell of Ad7, Ad5.F7, Ad5.F741S, Ad5.F41T, Ad5, or Ad5.F7F5. Membranes were probed with radiolabeled DNA fragments corresponding to the indicated fiber terminal exons. An Ad5 hexon-labeled DNA fragment was used as a control for MLTU viral products and has limited homology with Ad7 hexon.