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. 1971 Sep;21(3):443–454.

Studies on the IgA system of the horse

J P Vaerman, P Querinjean, J F Heremans
PMCID: PMC1408142  PMID: 5568324

Abstract

Equine serum and secretions were found to contain a protein which cross-reacted with an antiserum against human IgA, but not with antisera against any other human immunoglobulin. The physicochemical properties of equine IgA resembled those of human IgA. IgA was found to be the immunoglobulin having the highest secretion vs serum concentration ratio in equine lacteal and salivary secretions, and to be the protein produced by the majority of immunoglobulin-containing cells in the lamina propria of the equine intestine.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Acharya U. S., Rao S. S. Studies on horse antibodies. I. Development of antibody in different fractions of serum during immunization of horse with diphtheria toxoid. Indian J Biochem. 1966 Mar;3(1):33–37. [PubMed] [Google Scholar]
  2. Audibert F., Sandor G. Nature de la fraction antitoxine des immunsérums de cheval. C R Acad Sci Hebd Seances Acad Sci D. 1968 Jul 22;267(4):457–458. [PubMed] [Google Scholar]
  3. Bienenstock J. Immunoglobulins of the hamster. II. Characterization of the gamma A and other immunoglobulins in serum and secretions. J Immunol. 1970 May;104(5):1228–1235. [PubMed] [Google Scholar]
  4. Bistany T. S., Tomasi T. B., Jr Serum and secretory immunoglobulins of the rat. Immunochemistry. 1970 May;7(5):453–460. doi: 10.1016/0019-2791(70)90227-2. [DOI] [PubMed] [Google Scholar]
  5. Bourne F. J. IgA immunoglobulin from porcine serum. Biochem Biophys Res Commun. 1969 Jul 7;36(1):138–145. doi: 10.1016/0006-291x(69)90660-3. [DOI] [PubMed] [Google Scholar]
  6. Buchowicz I., Goch H., Zakrzewski K. Equine immunoglobulins: a comparison of molecular properties. Acta Biochim Pol. 1969;16(3):279–296. [PubMed] [Google Scholar]
  7. CRABBE P. A., CARBONARA A. O., HEREMANS J. F. THE NORMAL HUMAN INTESTINAL MUCOSA AS A MAJOR SOURCE OF PLASMA CELLS CONTAINING GAMMA-A-IMMUNOGLOBULIN. Lab Invest. 1965 Mar;14:235–248. [PubMed] [Google Scholar]
  8. Crabbé P. A., Bazin H., Eyssen H., Heremans J. F. The normal microbial flora as a major stimulus for proliferation of plasma cells synthesizing IgA in the gut. The germ-free intestinal tract. Int Arch Allergy Appl Immunol. 1968;34(4):362–375. doi: 10.1159/000230130. [DOI] [PubMed] [Google Scholar]
  9. Crandall R. B., Cebra J. J., Crandall C. A. The relative proportions of IgG-, IgAand IgM-containing cells in rabbit tissues during experimental trichinosis. Immunology. 1967 Feb;12(2):147–158. [PMC free article] [PubMed] [Google Scholar]
  10. Dorrington K. J., Rockey J. H. Studies on the conformation of purified human and canine gamma-A-globulins and equine gamma-T-globulin by optical rotatory dispersion. J Biol Chem. 1968 Dec 25;243(24):6511–6519. [PubMed] [Google Scholar]
  11. Ernst M. L., Arnon R., Sela M. Cleavage of horse immunoglobulin by cyanogen bromide. Immunochemistry. 1968 Nov;5(6):513–524. doi: 10.1016/0019-2791(68)90088-8. [DOI] [PubMed] [Google Scholar]
  12. FLEISCHMAN J. B., PORTER R. R., PRESS E. M. THE ARRANGEMENT OF THE PEPTIDE CHAINS IN GAMMA-GLOBULIN. Biochem J. 1963 Aug;88:220–228. doi: 10.1042/bj0880220. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Fateh-Moghadam A., Pichlymayr R., Jarosch von Schweder H. W., Knedel M. Isolierung immunologisch wirksamer gamma-Globuline eines Pferdeantihundelymphocytenserums. Klin Wochenschr. 1967 Jun 1;45(11):578–585. doi: 10.1007/BF01719815. [DOI] [PubMed] [Google Scholar]
  14. Genco R. J., Karush F., Tenenhouse H. S. Equine antihapten antibody. VI. Subunits of polyalanylated gamma-G(T)-immunoglobulin. Biochemistry. 1968 Jul;7(7):2462–2468. doi: 10.1021/bi00847a003. [DOI] [PubMed] [Google Scholar]
  15. Genco R. J., Yecies L., Karush F. The immunoglobulins of equine colostrum and parotid fluid. J Immunol. 1969 Sep;103(3):437–444. [PubMed] [Google Scholar]
  16. Grey H. M., Sher A., Shalitin N. The subunit structure of mouse IgA. J Immunol. 1970 Jul;105(1):75–84. [PubMed] [Google Scholar]
  17. HEREMANS J. F., VAERMAN J. P., VAERMAN C. STUDIES ON THE IMMUNE GLOBULINS OF HUMAN SERUM. II. A STUDY OF THE DISTRIBUTION OF ANTI-BRUCELLA AND ANTI-DIPHTHERIA ANTIBODY ACTIVITIES AMONG GAMMA-SS, GAMMA-IM AND GAMMA-1A-GLOBULIN FRACTIONS. J Immunol. 1963 Jul;91:11–17. [PubMed] [Google Scholar]
  18. Hill W. C., Cebra J. J. Horse anti-SI immunoglobulins. I. Properties of gamma-M-antibody. Biochemistry. 1965 Dec;4(12):2575–2584. doi: 10.1021/bi00888a005. [DOI] [PubMed] [Google Scholar]
  19. Johnson J. S., Vaughan J. H. Canine immunoglobulins. I. Evidence for six immunoglobulin classes. J Immunol. 1967 May;98(5):923–934. [PubMed] [Google Scholar]
  20. KLIMAN N. R., ROCKEY J. H., KARUSH F. VALENCE AND AFFINITY OF EQUINE NONPRECIPITATING ANTIBODY TO A HAPTENIC GROUP. Science. 1964 Oct 16;146(3642):401–403. doi: 10.1126/science.146.3642.401. [DOI] [PubMed] [Google Scholar]
  21. Klinman N. R., Rockey J. H., Frauenberger G., Karush F. Equine anti-hapten antibody. 3. The comparative properties of gamma G- and gammaA-antibodies. J Immunol. 1966 Apr;96(4):587–595. [PubMed] [Google Scholar]
  22. LAURELL C. B. ANTIGEN-ANTIBODY CROSSED ELECTROPHORESIS. Anal Biochem. 1965 Feb;10:358–361. doi: 10.1016/0003-2697(65)90278-2. [DOI] [PubMed] [Google Scholar]
  23. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  24. Lavergne M., Raynaud M., Iscaki S. Fixation du complément et induction de la réaction d'anaphylaxie cutanée passive (PCA) par les gammag-antitoxines de cheval. Ann Inst Pasteur (Paris) 1966 Mar;110(3 Suppl):155–160. [PubMed] [Google Scholar]
  25. Mach J. P., Pahud J. J. Secretory IgA, a major immunoglobulin in most bovine external secretions. J Immunol. 1971 Feb;106(2):552–563. [PubMed] [Google Scholar]
  26. Mancini G., Carbonara A. O., Heremans J. F. Immunochemical quantitation of antigens by single radial immunodiffusion. Immunochemistry. 1965 Sep;2(3):235–254. doi: 10.1016/0019-2791(65)90004-2. [DOI] [PubMed] [Google Scholar]
  27. Montgomery P. C., Bello A. C., Rockey J. H. N-Terminal sequences of equine and human immunoglobulin heavy chains. Biochim Biophys Acta. 1970 Feb 17;200(2):258–266. doi: 10.1016/0005-2795(70)90169-8. [DOI] [PubMed] [Google Scholar]
  28. Nash D. R., Vaerman J. P., Bazin H., Heremans J. F. Comparative molecular size of mouse IgA in different body fluids. Int Arch Allergy Appl Immunol. 1970;37(2):167–174. doi: 10.1159/000230230. [DOI] [PubMed] [Google Scholar]
  29. Nash D. R., Vaerman J. P., Bazin H., Heremans J. F. Identification of IgA in rat serum and secretions. J Immunol. 1969 Jul;103(1):145–148. [PubMed] [Google Scholar]
  30. Pahud J. J., Mach J. P. Identification of secretory IgA, free secretory piece and serum IgA in the ovine and caprine species. Immunochemistry. 1970 Aug;7(8):679–686. doi: 10.1016/0019-2791(70)90174-6. [DOI] [PubMed] [Google Scholar]
  31. Porter P., Allen W. D. Intestinal IgA in the pig. Experientia. 1970 Jan 15;26(1):90–92. doi: 10.1007/BF01900412. [DOI] [PubMed] [Google Scholar]
  32. Porter P., Noakes D. E., Allen W. D. Secretory IgA and antibodies to Escherichia coli in porcine colostrum and milk and their significance in the alimentary tract of the young pig. Immunology. 1970 Feb;18(2):245–257. [PMC free article] [PubMed] [Google Scholar]
  33. ROCKEY J. H., KLINMAN N. R., KARUSH F. EQUINE ANTIHAPTEN ANTIBODY. I. 7S BETA-2A- AND 1OS GAMMA-1- GLOBULIN COMPONENTS OF PURIFIED ANTI-BETA-LACTOSIDE ANTIBODY. J Exp Med. 1964 Oct 1;120:589–609. doi: 10.1084/jem.120.4.589. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Raynaud M., Iscaki S., Mangalo R. Séparation chromatographique des gamma G et gamma A antitoxines antidiphtériques de cheval. Ann Inst Pasteur (Paris) 1965 Oct;109(4):525–551. [PubMed] [Google Scholar]
  35. Raynaud M., Lavergne M., Iscaki S. Action inhibitrice des fragments 3 S monovalents d'anticorps (antitoxine antidiphtérique de cheval) sur l'hémagglutination passive. Ann Inst Pasteur (Paris) 1966 Mar;110(3 Suppl):218–236. [PubMed] [Google Scholar]
  36. Rockey J. H. Equine antihapten antibody. The subunits and fragments of anti-beta-lactoside antibody. J Exp Med. 1967 Feb 1;125(2):249–275. doi: 10.1084/jem.125.2.249. [DOI] [PMC free article] [PubMed] [Google Scholar]
  37. Sardesai S. K., Rao S. S. Immunoglobulins in hyperimmune horse serum. Indian J Biochem. 1968 Jun;5(2):46–49. [PubMed] [Google Scholar]
  38. Stechschulte D. J., Austen K. F. Immunoglobulins of rat colostrum. J Immunol. 1970 May;104(5):1052–1062. [PubMed] [Google Scholar]
  39. VAN DER Scheer J., Wyckoff R. W. ELECTROPHORETIC ANALYSES OF HYPERIMMUNE SERA. Science. 1940 May 17;91(2368):485–486. doi: 10.1126/science.91.2368.485. [DOI] [PubMed] [Google Scholar]
  40. Vaerman J. P., Arbuckle J. B., Heremans J. F. Immunoglobulin A in the pig. II. Sow's colostral and milk IgA: quantitative studies and molecular size estimation. Int Arch Allergy Appl Immunol. 1970;39(2-3):323–333. [PubMed] [Google Scholar]
  41. Vaerman J. P., Heremans J. F. Distribution of various immunoglobulin containing cells in canine lymphoid tissue. Immunology. 1969 Oct;17(4):627–633. [PMC free article] [PubMed] [Google Scholar]
  42. Vaerman J. P., Heremans J. F. Immunoglobulin A in the pig. I. Preliminary characterization of normal pig serum IgA. Int Arch Allergy Appl Immunol. 1970;38(6):561–572. doi: 10.1159/000230312. [DOI] [PubMed] [Google Scholar]
  43. Vaerman J. P., Heremans J. F. The immunoglobulins of the dog. II. The immunoglobulins of canine secretions. Immunochemistry. 1969 Nov;6(6):779–786. doi: 10.1016/0019-2791(69)90284-5. [DOI] [PubMed] [Google Scholar]
  44. Vaerman J. P., Heremans J. F., Van Kerckhoven G. Communications. Identification of IgA in several mammalian species. J Immunol. 1969 Dec;103(6):1421–1423. [PubMed] [Google Scholar]
  45. Weir R. C., Porter R. R., Givol D. Comparison of the C-terminal amino-acid sequence of two horse immunoglobulins IgG and IgG(T). Nature. 1966 Oct 8;212(5058):205–206. doi: 10.1038/212205a0. [DOI] [PubMed] [Google Scholar]
  46. Wofsy L., Klinman N. R., Karush F. Affinity labeling of equine anti-beta-lactoside antibodies. Biochemistry. 1967 Jul;6(7):1988–1991. doi: 10.1021/bi00859a016. [DOI] [PubMed] [Google Scholar]

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