FIG. 2.

Western blot analysis of Myb expression in murine and feline lymphoma cell lines. Twenty micrograms of protein aliquots was separated on SDS-polyacrylamide gels and subjected to Western blot analysis, using an anti-Myb mouse monoclonal antibody, which detects both the major (p75) and the minor (p89) exon 9A isoform of Myb. β-Actin expression was analyzed as a control for sample loading and integrity. Antibody complexes were visualized by enhanced chemiluminescence. A solid black line indicates the cell lines rearranged at Ahi-1 and fit-1.