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. 2003 Jan;77(2):1059–1068. doi: 10.1128/JVI.77.2.1059-1068.2003

FIG. 4.

FIG. 4.

(A) Diagrammatic structure of the c-myb locus in human, mouse, and cat genomes showing the location of neighboring genes. Retroviral insertion sites which have been physically mapped (feline genome) or located by BLAST searching of the draft genome sequence are also depicted. Horizontal single-headed arrows represent the orientations of the genes, and single-headed arrows with bent stems above the insertion sites represent the favored orientation of the integrated proviruses. The open boxes indicate annotated genes from the public domain human and murine sequences, with vertical lines representing the positions of individual exons. The feline genes are less well characterized and are shown in outline structure only. The AL136797 transcript is the longest corresponding transcript encompassing the annotated FLJ20069 gene, as shown in panel B. The symbol X within the feline genome represents a site where the restriction enzyme XhoI cuts. (B) Detailed diagram of the human FLJ20069 gene and its predicted gene product structure. The portion of the long AL136797 transcript corresponding to the annotated FLJ20069 gene is represented by a double-headed arrow. The orthologous murine sequences corresponding to this gene are currently annotated as two separate elements, Q9CVY1 and ENSMUSG00000037571 (“novel”) (grey box). AL136797 encompasses 25 exons, while the murine orthologues Q9CVY1 and ENSMUSG00000037571 contain 6 and 13 exons, respectively (top). Exons 3 to 7 of AL136797 (human) are homologous to exons 2 to 6 of murine Q9CVY1, while exons 11 to 22 of AL136797 are homologous with exons 1 to 13 of murine ENSMUSG00000037571. Diagonal lines drawn between the human and murine orthologues indicate regions of homology. The dotted line above exons 2 to 5 of murine ENSMUSG00000037571 indicates the area used to design RT-PCR primers for analysis of the expression of the murine orthologue of FLJ20069 (probe FLJ). The solid black line between Q9CVY1 and ENSMUSG00000037571 indicates an additional region of homology identified between the murine genome and exons 7 to 10 of human AL136797. Protein family motifs, including coiled-coil, WD-40, and SH3 domains, are depicted in the predicted amino acid structure of human AL136797 (bottom) and the relative positions of the amino acid matches to murine Q9CVY1 and ENSMUSG00000037571 are indicated by horizontal black lines.