FIG. 5.

Northern analysis of HBS1 expression in murine (A) and feline (B) lymphoma cell lines, and RT-PCR analysis of the expression of the murine orthologue of FLJ20069 (C). Total RNA samples (20 μg) were separated on formaldehyde-agarose gels and transferred to Northern blots which were probed with radiolabeled full-length murine HBS1 (A) or feline HBS1 exon 1 (B) cDNA fragments and GAPDH to control for RNA loading and integrity. For RT-PCR, 10 μl of each reaction mixture was separated by gel electrophoresis, transferred to a Southern blot, and probed with a radiolabeled murine FLJ20069 cDNA fragment (C). To control for sample integrity, cDNAs were also analyzed by RT-PCR with HPRT primers and probed with a radiolabeled murine HPRT fragment. 16iE and 16iL represent early- and late-passage p/m16i cells, respectively. A solid black line indicates cell lines rearranged at Ahi-1 and fit-1.